矿山酸性水中浸矿细菌的筛选及除铁效果

金属矿山 ›› 2014, Vol. 43 ›› Issue (02): 69-72.

矿山酸性水中浸矿细菌的筛选及除铁效果

陆洪省，刘月月，赵晓舒，王亚舒

山东科技大学化学与环境工程学院，山东青岛 266590

出版日期:2014-02-15 发布日期:2014-04-21
基金资助:
山东省博士后创新项目（编号：201201008）。

Isolation of Mineral Leaching Bacteria from Acid Mine Water and Study on Its Iron Removal Ability

Lu Hongsheng，Liu Yueyue，Zhao Xiaoshu，Wang Yashu

College of Chemical and Environmental Engineering，Shandong University of Science and Technology，Qingdao 266590，China

Online:2014-02-15 Published:2014-04-21

摘要/Abstract

摘要： 细菌浸矿在矿石综合利用和生态环境保护方面具有重要意义。从兖州煤矿酸性矿井水中筛选、分离到1株具有嗜酸、氧化Fe2+的浸矿细菌，编号SKDYJ-1。通过对其16S rDNA 基因序列分析，发现该菌株16S rDNA 基因序列与Pantoea agglomerans P1SAA（FJ611818）的同源性高达98% ，为嗜酸成团泛菌。对该菌株形态、生长特性、Fe2+氧化率进行了研究，结果表明：菌株 SKDYJ-1细胞呈杆状，革兰氏阴性，最适生长温度为30 ℃，在25 ℃、30 ℃以及35 ℃条件下培养，菌株SKDYJ-1对Fe²⁺的最大氧化率分别为2.79%、6.52% 和5.59%，具有较高的浸矿能力。

关键词: 酸性矿井水, 氧化Fe²⁺, 16S rDNA, 嗜酸成团泛菌

Abstract: Bacterial leaching of ore has important significance in comprehensive utilization of minerals and ecological environmental protection.A leaching strain named SKDYJ-1 with the capabilities of acidophilic，oxidizing Fe2+ was isolated from acid mine water of Yanzhou Coal Mine.Through analysis on its 16S rDNA gene sequences，it is found that 98% of the strain 16S rDNA gene sequences are homologous with Pantoea agglomerans P1SAA (FJ611818)，called eosinophils pantoea agglomerans.Its morphology，growth characteristics and Fe2+ oxidation were investigated.The results indicated that strain SKDYJ-1 was Gram-negative in the rod.The optimum growth temperature for this strain was 30 ℃.Under the culture temperatures of 25 ℃，30 ℃ and 35 ℃，the maximum oxidation rates of the strain SKDYJ-1 on Fe²⁺ showed as 2.79% ，6.52% and 5.59% respectively with a high leaching capacity.

Key words: Acid mine water, Fe²⁺ oxidation, 16S rDNA, Pantoea

陆洪省, 刘月月, 赵晓舒, 王亚舒. 矿山酸性水中浸矿细菌的筛选及除铁效果[J]. 金属矿山, 2014, 43(02): 69-72.

LU Hong-Sheng, LIU Yue-Yue, ZHAO Xiao-Shu, WANG Ya-Shu. Isolation of Mineral Leaching Bacteria from Acid Mine Water and Study on Its Iron Removal Ability[J]. Metal Mine, 2014, 43(02): 69-72.

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