关键词: 硫酸盐还原菌, 煤矸石, 酸化污染, 微生物修复

Abstract: The high salinity acid drainage caused by the oxidation of coal gangue are one of the pollution sources that seriously harm the environmental quality of coal mine area. The use of sulfate-reducing bacteria for biological sulfur fixation can effectively control the acid production and pollution caused by the oxidation of coal gangue. In view of the problem that most of the known sulfate reducing bacteria are strict anaerobic bacteria,depending on external carbon source,and difficult to be applied to open coal gangue mountain,a facultative anaerobic sulfate reducing bacteria was isolated and screened from the soil around the acid coal gangue yard. The 16S rDNA gene sequence,morphology,physiological and biochemical characteristics of isolated strain were investigated,and the column leaching experiments was used to analyze the repair effect of the strain on coal gangue acidification pollution. The results showed that the strain had 99. 93% homology with Bacillus subtilis,and its shape was rod-shaped,with size (0. 4~ 0. 6) μm×0. 2 μm. The optimal growth temperature range of the strain was 25~ 35 ℃ ,and it grew well with pH of 4~ 8. In the absence of any additional carbon source,the pH value of the acidified coal gangue was raised from 3. 09 to 4. 62 after inoculation of the strain for 18 days,and 48. 25% sulfate ions and more than 88. 40% heavy metal were removed,effectively controlling the generation of high-salt acidic wastewater in the coal gangue yard.

Key words: sulfate-reducing bacteria,coal gangue,acidification pollution,microbial remediation