As(Ⅲ)和As(Ⅴ)胁迫下浸矿细菌胞外多糖的变化特征

金属矿山 ›› 2016, Vol. 45 ›› Issue (05): 81-84.

As(Ⅲ)和As(Ⅴ)胁迫下浸矿细菌胞外多糖的变化特征

富瑶¹，高鹭¹，杨洪英²，佟琳琳²，杨宇¹，胡晓静¹

1.辽宁出入境检验检疫局，辽宁大连 116001；2.东北大学材料与冶金学院，辽宁沈阳 110819

出版日期:2016-05-13 发布日期:2016-08-18
基金资助:
基金项目国家自然科学基金项目(编号：51304047）。

Changes Characteristics of Extracellular Polysaccharide in Leaching Bacteria under As(Ⅲ) and As（Ⅴ) Stress

Fu Yao¹，Gao Lu¹，Yang Hongying²，Tong Linlin²，Yang Yu¹，Hu Xiaojing¹

1.Liaoning Entry-Exit Inspection and Quarantine Bureau,Dalian 116001,China；2.School of Materials Science & Metallurgy，Northeastern University,Shenyang 110819,China

Online:2016-05-13 Published:2016-08-18

摘要/Abstract

摘要： 为考察浸矿细菌胞外多糖含量与砷离子的浓度和价态以及与浸矿细菌生长指标的关系，进而明确浸矿细菌抵御砷离子的生物对策，研究了不同浓度的As(Ⅲ)和As(Ⅴ)胁迫时，浸矿细菌胞外多糖的行为变化；通过测定菌液的pH、Eh、Fe²⁺浓度、菌数以及胞外多糖的含量，分析了各测定指标的相关性和线性关系。研究表明：As(Ⅲ)胁迫下，菌液的胞外多糖产量迅速增长，增幅超过34%，As(Ⅲ)的胁迫作用强于As(Ⅴ)；As(Ⅲ)胁迫使菌液胞外多糖含量的变异中有63.4%与Eh线性相关、有61.6%与Fe²⁺浓度线性相关、有71.4%与菌数线性相关；浸矿细菌通过代谢调控菌液的理化指标来调节胞外多糖的含量，进而抵抗砷离子的胁迫。

关键词: 浸矿细菌, 含砷矿石, 砷离子胁迫, 胞外多糖

Abstract: The behavior changing of extracellular polysaccharide in leaching bacteria was studied under the different concentrations of As(Ⅲ) and As(Ⅴ) stress，in order to investigate the relationship between extracellular polysaccharide in leaching bacteria，concentration and valence of arsenic ion，and increase indexes of leaching bacteria，so as to find out biological strategies of leaching bacteria against arsenic ion.The pH，Eh，Fe²⁺ concentration，the number of bacteria and the content of extracellular polysaccharide were measured to analysis the correlation and the linear relationship between them.The results show that under As(Ⅲ) stress，the production of extracellular polysaccharide in the bacterial liquid increases over 34%，As(Ⅲ) stress effect is stronger than As(Ⅴ)，and the extracellular polysaccharide content is linear correlation with Eh，Fe²⁺ concentration and the number of bacteria at level of 63.4%，61.6% and 71.4% respectively.The results also indicate that leaching bacteria can regulate and control the growth indexes of the bacterial liquid to adjust the amount of extracellular polysaccharide，thus to resist arsenic ion stress.

Key words: Bacteria metallurgy, Arsenic ores, Arsenic ion stress, Extracellular polysaccharide

富瑶, 高鹭, 杨洪英, 佟琳琳, 杨宇, 胡晓静. As(Ⅲ)和As(Ⅴ)胁迫下浸矿细菌胞外多糖的变化特征[J]. 金属矿山, 2016, 45(05): 81-84.

FU Yao, GAO Lu, YANG Hong-Ying, TONG Lin-Lin, YANG Yu, HU Xiao-Jing. Changes Characteristics of Extracellular Polysaccharide in Leaching Bacteria under As(Ⅲ) and As（Ⅴ) Stress[J]. Metal Mine, 2016, 45(05): 81-84.

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