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Metal Mine ›› 2011, Vol. 40 ›› Issue (09): 82-86.

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Bioleaching of a Low Grade  Copper Sulphide Ore by Mixed Culture

Han Tao1,2，Yin Huaqun1,2，Liu Yi1,2，Shen Li1,2，Liang Yili1,2，Liu Xueduan1,2   

  1. 1.School of Minerals Processing and Bioengineering，Central South University；2.Key Laboratory of Biometallurgy，Ministry of Education
  • Online:2011-09-13 Published:2011-09-19

Abstract: The bioleaching of a kind of low grade sulphide ores was studied used a mixed culture of acidophilic microorganisms in a stirred tank reactor.RT-PCR（real-time fluorescence quantitative polymerase chain reaction) was used to identify the structure of the communities during bioleaching process.The results revealed that the bioleaching efficiency of Cu up to 85.66% in 20 d，and the acid-leaching without inocula only 24.43%.The bioleaching efficiency was up to 79.50% after 12 d bioleaching.At.ferrooxidans was the dominant with increase of Eh and decrease of Fe2+ during the leaching time from 2 to 12 d.After 12 d,At.thiooxidans gradually increased to replace At.ferrooxidans with the leaching rate decreased.Moreover，Mantel test indicated that the structure of the community were significantly correlated with Cu and Fe.

Key words: Low grade copper sulphide ores, Tank bioleaching, Mixed culture, RT-PCR