Abstract: The valuable minerals of copper-lead-zinc poly-metallic sulfide ore have complex dissemination with uneven grain size.Beneficiation experiment on copper，lead，zinc by using one grinding and potential controlled preferential flotation was conducted.Results indicated that using SN-9#+ aniline aerofloat as collector，Na₂SiO₃+ZnSO₄+CMC as inhibitor at potential of -35 mV，pH=9.1 for copper preferential flotation，then using SN-9#+ aniline aerofloat as collector at potential of -225.6 mV，pH=11.4 for lead flotation，finally using copper sulfate as activating agent，ethyl xanthate as collector in zinc flotation，copper concentrate with copper grade of 23.68% and recovery of 85.61%，lead concentrate with lead grade of 51.26% and recovery of 70.68%，and zinc concentrate with zinc grade of 52.13% and recovery of 82.13% was obtained respectively.

Key words: Preferential flotation, Potential controlled, Pulp regulator CaO, Combined collector SN-9#+ aniline aerofloat, Combined inhibitor Na₂SiO₃+ZnSO₄+CMC