Abstract: To select the Acidithiobacillus ferrooxidans and explore its adsorption performance on pyrite surface，the strain APY was separated from a acid pit drainage of Ganzhou，Jiangxi.The morphology，growth characteristics and 16S rDNA sequences of the strain were analyzed.Furthermore，the adsorption characteristics of the strain on pyrite surface were explored.The result indicated that，the strain APY was in the same branch of the phylogenetic tree with A.ferrooxidans strain (Acidithiobacillus ferrooxidans CK and BGL-2)，with coverage degree and similarity degree more than 98%.So the strain APY was identified as A.ferrooxidans.The optimum growth conditions of APY strain were as follows:①The volume ratio of bacteria seed liquid to culture medium was 10%; ②initial liquid medium pH was 2.0; ③rotational speed of air bath shaker was 180 r/min; ④the culture temperature was 30 ℃.Among them，pH is the limiting factor for the growth of APY strain.The strain APY adsorbed on the surface of the pyrite was a rapid process，and the adsorption number on surface of the pyrite were of up to 1.5×108 cell/mL when the pH was 2.0.

Key words: APY strain, Acidithiobacillus ferrooxidans, Culture characteristics, Surface adsorption, Pyrite