Abstract: Using fatty acid anionic collector to float iron minerals requires pulp heated to ensure the collecting.It not only led to a large number of energy consumption but also will do harm to the environment.Thus，novel cationic collector DYP used in normal temperature was developed by Northeastern University.In order to study the reverse flotation separation effect on iron minerals，flotation experiments on single quartz，hematite，magnetite and artificial mixed minerals were conducted.The results indicated that：floatability of the three single minerals is as follows quartz>hematite>magnetite.The optimum dosage of DYP is 50 mg/L and the recovery rate of quartz is 97.33%，while magnetite and hematite are 6.50% and 4.33%，respectively.DYP has good floatability and can be used in iron mineral reverse flotation.Analysis on chemical equilibrium of collector DYP in solution，zeta potential of quartz surface and floatability to DYP revealed that DYP collecting ability on quartz is the result of synergy of both the electrostatic adsorption and hydrogen bonding adsorption，and hydrogen bonding interaction is stronger than that of the electrostatic adsorption.

Key words: Flotation, Mono-mineral, Artificial mixed ore, Cationic collector DYP