Abstract: The separation of copper from microbial leaching solution of waste printed circuit boards by extraction was studied.The results showed that: Using N902 as the extraction agent，a copper extraction from leaching solution can be selectively realized.Under the conditions of the 10% extraction agent，extract phase of 1∶1 and extract time of 5 min，the copper extraction rate can be up to 99.51% with Cu and Fe separation factor of 2 058; With sulfuric acid solution as the stripping agent，back-extraction on the loading organic phase is carried out.Under the conditions of the sulfuric acid concentration of 1.8 mol / L，back extraction phase rate of 1∶1，at back-extraction time of 5 min，the back-extraction rate of copper can be up to 93.57%.  

Key words: Waste printed circuit boards, Microbial leaching liquid, Copper, Extraction