某低品位硫化铜矿混合菌浸出研究

金属矿山 ›› 2011, Vol. 40 ›› Issue (09): 82-86.

某低品位硫化铜矿混合菌浸出研究

韩涛^1,2，尹华群^1,2，刘毅^1,2，申丽^1,2，梁伊丽^1,2，刘学端^1,2

1.中南大学资源加工与生物工程学院；2.生物冶金教育部重点实验室

出版日期:2011-09-13 发布日期:2011-09-19
基金资助:
* 国家重点基础研究发展计划（973计划）项目（编号：2010CB630901）。

Bioleaching of a Low Grade Copper Sulphide Ore by Mixed Culture

Han Tao^1,2，Yin Huaqun^1,2，Liu Yi^1,2，Shen Li^1,2，Liang Yili^1,2，Liu Xueduan^1,2

1.School of Minerals Processing and Bioengineering，Central South University；2.Key Laboratory of Biometallurgy，Ministry of Education

Online:2011-09-13 Published:2011-09-19

摘要/Abstract

摘要： 利用混合菌对低品位硫化铜矿进行槽浸研究，并通过实时荧光定量PCR（RT-PCR）技术监测浸出过程中菌群的动态变化。研究结果表明，多种浸矿菌共同作用20 d，硫化铜矿中铜的生物浸出率达到85.66%，而酸浸仅为24.43%。在生物浸出的2～12 d，At.ferrooxidans是主要的浸矿菌，浸出液中Fe²⁺浓度快速下降、Eh上升，促进硫化矿的快速分解，Cu的浸出率达到79.50%；12 d以后，混合菌中的优势种逐渐被At.thiooxidans取代，浸出速度逐渐降低。此外，Mantel test结果表明，浸出液中Cu和Fe与微生物群落结构显著相关。

关键词: 低品位硫化铜矿, 槽浸, 混合菌, 实时荧光定量PCR

Abstract: The bioleaching of a kind of low grade sulphide ores was studied used a mixed culture of acidophilic microorganisms in a stirred tank reactor.RT-PCR（real-time fluorescence quantitative polymerase chain reaction) was used to identify the structure of the communities during bioleaching process.The results revealed that the bioleaching efficiency of Cu up to 85.66% in 20 d，and the acid-leaching without inocula only 24.43%.The bioleaching efficiency was up to 79.50% after 12 d bioleaching.At.ferrooxidans was the dominant with increase of Eh and decrease of Fe2+ during the leaching time from 2 to 12 d.After 12 d,At.thiooxidans gradually increased to replace At.ferrooxidans with the leaching rate decreased.Moreover，Mantel test indicated that the structure of the community were significantly correlated with Cu and Fe.

Key words: Low grade copper sulphide ores, Tank bioleaching, Mixed culture, RT-PCR

韩涛, 尹华群, 刘毅, 申丽, 梁伊丽, 刘学端. 某低品位硫化铜矿混合菌浸出研究[J]. 金属矿山, 2011, 40(09): 82-86.

HAN Tao, YIN Hua-Qun, LIU Yi, SHEN Li, LIANG Yi-Li, LIU Xue-Duan. Bioleaching of a Low Grade Copper Sulphide Ore by Mixed Culture[J]. Metal Mine, 2011, 40(09): 82-86.

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