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金属矿山 ›› 2016, Vol. 45 ›› Issue (09): 117-121.

• 矿物工程 • 上一篇    下一篇

嗜酸氧化亚铁硫杆菌APY的生长特性及其在黄铁矿表面的吸附

余水静1,2,彭涛1,2,程素1,2,陈江安1,3,邱廷省1,3,4   

  1. 1.江西理工大学资源与环境工程学院,江西 赣州 341000;2.江西省矿冶环境污染控制重点实验室,江西 赣州 341000;3.江西省矿业工程重点实验室,江西 赣州 341000;4.江西理工大学,江西 赣州 341000
  • 出版日期:2016-09-15 发布日期:2016-10-18
  • 基金资助:

    基金项目 国家自然科学基金项目(编号:51474114),江西省科技厅科技支撑计划项目(编号:20121BBG70004)。

Culture Characteristics of Acidithiobacillus Ferrooxidans APY and Its Adsorption on Pyrite Surface

Yu Shuijing1,2,Peng Tao1,2,Cheng Su1,2,Chen Jiang′an1,3,Qiu Tingsheng1,3,4   

  1. 1.School of Resources and Environment Engineering,Jiangxi University of Science and Technology,Ganzhou 341000,China;2.Jiangxi Key Laboratory of Environmental Pollution Control of Mining and Metallurgy,Ganzhou 341000,China;3.Jiangxi Key Laboratory of Mining Engineering,Ganzhou 341000,China;4.Jiangxi University of Science and Technology,Ganzhou 341000,China
  • Online:2016-09-15 Published:2016-10-18

摘要: 为了筛选嗜酸氧化亚铁硫杆菌并探明其在黄铁矿表面的吸附性能,从赣州某矿山酸性矿坑水中分离得到APY菌,对该菌形态、生长特性和16S rDNA序列进行了分析,并对该菌在黄铁矿表面的吸附特性进行了研究。结果表明,APY菌与嗜酸氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans CK和BGL-2)处在系统发育树的同一分支,覆盖度和相似度均超过98%,判断APY菌为嗜酸氧化亚铁硫杆菌;APY菌适宜的生长条件为:①菌液与培养基体积比为10%;②培养液的初始pH=2.0;③恒温气浴摇床转速为180 r/min;④适宜的培养温度为30 ℃,其中pH为APY菌生长的限制性影响因素。APY菌在黄铁矿表面的吸附速度快,在pH=2.0时,20 min混合液中就有约1.5×108 cell/mL的APY菌吸附在黄铁矿表面。

关键词: APY菌, 嗜酸氧化亚铁硫杆菌, 培养特性, 表面吸附, 黄铁矿

Abstract: To select the Acidithiobacillus ferrooxidans and explore its adsorption performance on pyrite surface,the strain APY was separated from a acid pit drainage of Ganzhou,Jiangxi.The morphology,growth characteristics and 16S rDNA sequences of the strain were analyzed.Furthermore,the adsorption characteristics of the strain on pyrite surface were explored.The result indicated that,the strain APY was in the same branch of the phylogenetic tree with A.ferrooxidans strain (Acidithiobacillus ferrooxidans CK and BGL-2),with coverage degree and similarity degree more than 98%.So the strain APY was identified as A.ferrooxidans.The optimum growth conditions of APY strain were as follows:①The volume ratio of bacteria seed liquid to culture medium was 10%; ②initial liquid medium pH was 2.0; ③rotational speed of air bath shaker was 180 r/min; ④the culture temperature was 30 ℃.Among them,pH is the limiting factor for the growth of APY strain.The strain APY adsorbed on the surface of the pyrite was a rapid process,and the adsorption number on surface of the pyrite were of up to 1.5×108 cell/mL when the pH was 2.0.

Key words: APY strain, Acidithiobacillus ferrooxidans, Culture characteristics, Surface adsorption, Pyrite