Abstract: Experimental research is made on the effect of size, pulp concentration, acidity, inoculation amount and initial Fe2⁺ concentration on chalcopyrite leaching with T.f in shaking flask at a low temperature of 15℃ by orthogonal method. The test result indicates that the initial Fe²⁺ concentration has the most evident effect on the bioleaching process of copper and the optimal conditions at a temperature of 15℃ are: initial Fe²⁺ concentration of 6g/L, pH value of 2.0, inoculation amount of 15%, pulp concentration of 15% and particle size of 200 mesh.

Key words: Chalcopyrite, Low temperature, Bioleaching