Abstract: Acidithiobacillus thiooxidans are often used in the biological hydrometallurgy and metal-containing sludge treatment fields, but their natural bacterial strains have a limited acid-producing capacity. In this experiment, the improvement of the acid-producing capacity of the natural Acidithiobacillus thiooxidans separated from the acid drainage of a mine was studied, including the effect of different initial pH values and energy source of substrate on the acid-producing capacity. Meanwhile, microwave and ultraviolet induction breeding were adopted to further improve the acid-producing capacity of Acidithiobacillus thiooxidans.

Key words: Acidithiobacillus thiooxidans, Inducement, Acid-producing capacity