Abstract: Bacterial leaching of ore has important significance in comprehensive utilization of minerals and ecological environmental protection.A leaching strain named SKDYJ-1 with the capabilities of acidophilic，oxidizing Fe2+ was isolated from acid mine water of Yanzhou Coal Mine.Through analysis on its 16S rDNA gene sequences，it is found that 98% of the strain 16S rDNA gene sequences are homologous with Pantoea agglomerans P1SAA (FJ611818)，called eosinophils pantoea agglomerans.Its morphology，growth characteristics and Fe2+ oxidation were investigated.The results indicated that strain SKDYJ-1 was Gram-negative in the rod.The optimum growth temperature for this strain was 30 ℃.Under the culture temperatures of 25 ℃，30 ℃ and 35 ℃，the maximum oxidation rates of the strain SKDYJ-1 on Fe²⁺ showed as 2.79% ，6.52% and 5.59% respectively with a high leaching capacity.

Key words: Acid mine water, Fe²⁺ oxidation, 16S rDNA, Pantoea