Abstract: Gold mainly exists in form of sulfide-wrap minerals in a finely micro-disseminated gold deposit contains sulfur in Guizhou. Gold-bearing sulfide minerals are fine disseminated and closely associated with the gangue minerals. Fine grinding was needed to make the sulfide minerals to be liberated，while the gangue mineral are mainly carbonate or clay minerals，easily to slime. Fine grinding will produce large amount of slime，reduce the flotation index，so the two stage grinding stage flotation process was conducted. The results indicated that，at the grinding fineness of 80% -0.074 mm，using (NH₄)2SO₄ and CuSO₄ as activator，butyl xanthate and butyl ammonium aerofloat as collector，2# oil as frother，via one stage flotation of one roughing-one cleaning，first stage flotation tailing ground to 95% passing 0.074 mm，via two stage flotation of one roughing-three cleaning-three scavenging，bulk concentrate with gold grade of 50.79% and recovery of 88.31% was obtained. The results can provide technique basis for the development and utilization of the ore.

Key words: Sulfide ore associated gold, Sliming, Two-stage grinding and flotation