Abstract: With its rapid development, molecular biological technology has been more and more applied in the research of bio-leaching microorganisms. DNA G+C mol% determination and DNA/DNA hybridization are common molecular biological methods in the taxonomic identification. PCR technology and the analysis 16S rRNA gene sequence based on this are also widely used in the taxonomic identification,Meanwhile,RFLP and RAPD techniques have also played an important role in the taxonomic identification of bioleaching microorganisms.In addition,molecular biological technology has been used to reveal the microbial community composition and succession of bioleaching system, with the technologies such as DGGE, SSCP, ESP, FISH, gene bank and pulsed field get electrophoresis playing a great role.

Key words: Bioleaching microorganisms, Molecular biological technology, Biological metallurgy