Abstract: The paper describes the effect of pH value, culture time and inoculation amount on the growth of Thiobacillus Ferrooxidans. The measurement of the results of coal desulphurization by this bacterium shows that at an initial pH = 2 and an inoculation amount of 15% in 9K culture medium, the concentration of Thiobacillus Ferrooxidans after 5d growth reached 108 /mL, and 10 days later, the pyrite removal rate reached 52.29% and the total sulfur removal rate, 37.75%.

Key words: Thiobacillus Ferrooxidans, Removal rate, Potential