关键词: 白腐真菌, 生物絮凝剂, 絮凝条件优化, 煤泥水, 正交试验

Abstract: Through to the white-rot fungus's biology raise characteristic research, has obtained the best raise condition：In rotational speed 160 r/min, the temperature 30 ℃, rice husk Lixivium 90 mL, glucose mass concentration 35 g/L, under the pH value 3.5 conditions, the fresh growing trend is best; Simultaneously has analyzed in the slime wate the granulometric composition, discovered its granularity is smaller than the 0.045 mm content to account for 55.93%, but is bigger than 0.25 mm the content only to account for 3.75%, explained in the coal spate the fine grain content are more, depends on own gravity to subside with difficulty; Discussed the pH value, the biological flocculant amount used, has stirred the way and the metallic ion and so on the single factor to the coal spate flocculation effect influence, further optimized this fungus through the orthogonal experiment to produce the flocculant to the coal spate flocculation effect condition, finally indicated：When the slime wate mass concentration 27.42 g/L, helps congeals the medicinal preparation (CaCl₂) mass concentration 5.0 g/L, uses two sections of types to stir (the first section：Mixing speed 60 r/min, stirs time 180 s; Second section：When mixing speed 180 r/min, stirs time 60 s), the pH value 11 and flocculant volume concentration 15 mL/L, its flocculation activeness is best, may reach 98.71%; Further tests indicated that, the flocculation active material major part exists to the extracellular, it is the extracellular secretion which the mycelium cell produces in the fermentative process.This flocculant has a stronger thermostability, the many kinds of positive ions all are helpful to this flocculant to congeal the function, and divalent cation Ca²⁺ the flocculant which produces to the white-rot fungus helps congeals the effect to be remarkable, it has the good application prospect.

Key words: White-rot fungus, Bioflocculant, Flocculation condition optimization, Slime water, Orthogonal test