Abstract: There is 0.16% copper,4.43% zinc in a copper-zinc sulfide ore from Yunnan.Copper and zinc were both mainly in form of sulfide ore，and the oxidation degree is lower.In order to provide technology basis for the development and utilization of the ore，the flotation tests were carried out.The results indicated that，at the grinding fineness of 78% passing 74 μm，with OL-ⅡA as collector for preferential flotation of copper，via one roughing，three cleaning，two scavenging process(reground concentrate of first stage copper cleaning to 94% passing 38 μm)，tailings of copper with X-43 as activator，butyl xanthate as collector for zinc flotation(reground concentrate of first stage zinc cleaning to 91% passing 45 μm)，copper concentrate with 18.52% Cu and recovery of 53.89%，zinc concentrate with 47.10% Zn and recovery of 88.74% was obtained.The results can provide technique basis for development and utilization of the ore.

Key words: Copper-zinc sulfide ore, Preferential flotation, Separation of copper and zinc